What is the Difference Between Trizol and Trizol LS?

The main difference between TRIzol and TRIzol LS is the concentration of the components. TRIzol LS Reagent is slightly more concentrated than TRIzol Reagent, allowing it to accommodate the processing of more liquid samples per unit of reagent. Consequently, the ratio of TRIzol LS to the sample is 3:1, while the ratio of TRIzol to the sample is 10:1. Both reagents are composed of guanidium thiocyanate and are used for the extraction of DNA, RNA, and proteins from living tissues.

Another difference between the two reagents is their color. TRIzol LS Reagent is slightly darker, with a maroonish-red color, compared to the lighter pink color of TRIzol Reagent. Both reagents can be used for the simultaneous isolation of total RNA, DNA, and proteins from various sample types, including liquid samples such as serum and virus preparations.

Comparative Table: Trizol vs Trizol LS

Trizol and Trizol LS are both reagents composed of guanidium thiocyanate, which is a chaotropic agent used in the extraction of nucleic acids and proteins. However, there are some differences between the two:

Both Trizol and Trizol LS are composed of guanidium thiocyanate, act as chaotropic agents in the extraction of proteins and nucleic acids, cause effective protein denaturation, are highly sensitive to light, and are stored in amber bottles. Trizol LS is a concentrated form of Trizol, which increases the accuracy of extraction and speeds up the extraction reaction.