What is the Difference Between Template and Coding Strand?

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The template and coding strands are two complementary strands of DNA that encode genetic information. They differ in their properties and functions:

  • Coding Strand: This strand determines the correct nucleotide sequence of mRNA and is also known as the sense strand or plus strand. The coding strand contains codons, while the non-coding strand contains anticodons. It serves as a template for producing complementary RNA. The coding strand is directed in the 3' to 5' direction.
  • Template Strand: This strand acts as a base for mRNA transcription and is also known as the antisense strand or minus strand. The template strand guides the RNA polymerase to form mRNA with a similar sequence. It is directed in the 5' to 3' direction. The template strand does not have any complementary sequence.

In summary, the main differences between the coding strand and template strand are their roles in transcription, their complementary sequences, and their directions.

Comparative Table: Template vs Coding Strand

The main differences between the coding strand and the template strand are as follows:

Coding Strand Template Strand
Also known as the sense strand or plus strand. Also known as the antisense strand or minus strand.
Determines the correct nucleotide sequence of mRNA. Acts as a base for mRNA transcription.
Does not take part in the transcription process. Takes part in the transcription process, helping in the formation of mRNA.
Directed in the 3' to 5' direction. Directed in the 5' to 3' direction.
Has a complementary nucleotide sequence. Does not have any complementary sequence.