What is the Difference Between SDS Page and Western Blot?
🆚 Go to Comparative Table 🆚SDS-PAGE and Western Blot are both techniques used in protein analysis, but they serve different purposes and have distinct methods of operation.
SDS-PAGE (Sodium Dodecyl Sulfate Polyacrylamide Gel Electrophoresis)
- SDS-PAGE is a gel electrophoresis technique used for protein separation.
- It separates proteins based on their mass.
- Commonly used in biochemistry, genetics, forensics, and molecular biology.
- Proteins are run on a gel made up of SDS and polyacrylamide.
Western Blot (Immunoblotting)
- Western Blot is an analytical technique used to identify the presence of a specific protein within a complex mixture of proteins.
- It involves several key steps: gel electrophoresis, blotting, and hybridization.
- The goal is to identify a particular antigen within a complex mixture of proteins using a specific antibody.
- SDS-PAGE is often used as the first step in the procedure to separate the protein of interest.
In summary, SDS-PAGE is a technique used to separate proteins based on their mass, while Western Blot is an analytical technique to identify the presence of a specific protein within a complex mixture of proteins. SDS-PAGE is often used as the first step in the Western Blot procedure to separate the protein of interest.
Comparative Table: SDS Page vs Western Blot
Here is a table comparing the differences between SDS-PAGE and Western Blot:
| Feature | SDS-PAGE | Western Blot |
|---|---|---|
| Definition | SDS-PAGE is an electrophoresis method that separates proteins by mass. | Western blot is an analytical technique to identify the presence of a specific protein within a sample. |
| Purpose | Separates proteins based on their mass. | Identifies the presence of a specific protein within a sample. |
| Technique | Polyacrylamide gel electrophoresis with proteins denatured by SDS (Sodium dodecyl sulfate). | Gel electrophoresis is usually the first step in the Western blot procedure to separate proteins. |
| Sample | Proteins are separated in a gel matrix. | Proteins are separated in a gel matrix and then transferred to a membrane for further analysis. |
| Electrophoresis | Separation is based on the difference in size between proteins. | Separation involves transferring proteins to a membrane after gel electrophoresis. |
| Molecular Weight | Displayed molecular weight is affected by N-terminus charge and presence of an acidic peptide. | Molecular weight of the protein of interest is estimated by comparing it to known standards. |
SDS-PAGE is commonly used in Western blot procedures to separate proteins based on their mass. Once the proteins are separated by gel electrophoresis, they are transferred to a membrane for further analysis, such as identifying the presence of a specific protein within the sample.
- Elisa vs Western Blot
- Northern Southern vs Western Blotting
- Gel Electrophoresis vs SDS Page
- SDS Page vs Native Page
- Serum Protein Electrophoresis vs Immunofixation
- Native vs Denaturing Gel Electrophoresis
- Direct vs Indirect ELISA
- Sandwich Elisa vs Competitive Elisa
- Immunocytochemistry vs Immunohistochemistry
- In Situ Hybridization vs Immunohistochemistry
- Biochemical vs Cell Based Assays
- Gel vs Paper Electrophoresis
- Capillary Electrophoresis vs Gel Electrophoresis
- Flow Cytometry vs Immunohistochemistry
- Antiserum vs Antibody
- Atomic Absorption Spectroscopy vs UV Visible Spectroscopy
- Direct vs Indirect Immunofluorescence
- Monoclonal Antibodies vs Polyclonal Antibodies
- Immunofluorescence vs Immunohistochemistry