What is the Difference Between Klenow and T4 DNA Polymerase?
🆚 Go to Comparative Table 🆚The main difference between Klenow and T4 DNA polymerase lies in their origins and temperature requirements. Here are the key differences:
- Origin: Klenow fragment is a large fragment of E. coli DNA polymerase 1, while T4 DNA polymerase is the DNA polymerase 1 of bacteriophage T4.
- Temperature: Klenow fragment can be used at 25°C or room temperature, but T4 DNA polymerase must be used at 12°C due to its robust exonuclease.
Both Klenow and T4 DNA polymerase are prokaryotic polymerases with 5′→3′ polymerase and 3′→5′ exonuclease activity. They lack 5′→3′ exonuclease activity, making them suitable for many applications in molecular biology. Both enzymes require a template and a primer to catalyze the synthesis of DNA and can be heat inactivated at 75°C.
Comparative Table: Klenow vs T4 DNA Polymerase
Klenow and T4 DNA polymerases are both used in molecular biology for DNA synthesis, but they have some differences. Here is a table comparing their characteristics:
| Characteristic | Klenow Fragement | T4 DNA Polymerase |
|---|---|---|
| Origin | E. coli DNA Polymerase 1 | Bacteriophage T4 DNA Polymerase 1 |
| Structure | Large fragment of E. coli DNA Polymerase 1 | Separate enzyme |
| 5'→3' Polymerase Activity | Present | Present |
| 3'→5' Exonuclease Activity | Present | Present |
| 5'→3' Exonuclease Activity | Absent | Absent |
| Optimal Temperature | 37°C | 37°C |
| Error Rate | 1 x 10^-6 | 1 x 10^-6 |
| Blunt Ends | Yes | Yes |
Both Klenow and T4 DNA polymerases have 5'→3' polymerase and 3'→5' exonuclease activity, but they lack 5'→3' exonuclease activity, making them more suitable for many applications in molecular biology. They are both used in various techniques, such as DNA sequencing, nick translation, and mutagenesis.
- Klenow Fragment vs DNA Polymerase 1
- Taq Polymerase vs DNA Polymerase
- DNA Polymerase vs RNA Polymerase
- T4 DNA Ligase vs E Coli DNA Ligase
- DNA Ligase vs DNA Polymerase
- DNA Polymerase 1 2 vs 3
- Phusion vs Taq Polymerase
- Polymerase vs Primase
- T4 vs T7 DNA Ligase
- DNA Dependent DNA Polymerase vs DNA Dependent RNA Polymerase
- Taq vs Pfu Polymerase
- Prokaryotic vs Eukaryotic RNA Polymerase
- RNA Polymerase Core vs Holoenzyme
- PCR vs DNA Replication
- RNA Polymerase I II vs III
- Gene Cloning vs PCR
- Oligonucleotide vs Polynucleotide
- T4 vs Lambda Phage
- Proteinase K vs Protease