What is the Difference Between Kinetic and End Point Reaction?

The main difference between kinetic and end point reactions lies in how measurements are taken during the course of the reaction. Here are the key differences between the two methods:

Kinetic Reaction: In this method, multiple measurements are taken over the course of the reaction. The difference in absorbance between two points during the progression of the reaction is measured. The reaction time is usually short to avoid any danger of enzyme degradation. This method is useful for enzyme analysis in clinical chemistry.
End Point Reaction: In this method, a final measurement is taken to measure the total amount of substrate/products. The reaction time is typically longer compared to the kinetic method, usually around 5 to 15 minutes. The absorbance of the sample increases with time and reaches a stable value, marking the end point of the reaction. This method is also used in clinical chemistry to determine the total amount of analytes consumed in the reaction.

In summary, the kinetic reaction method focuses on measuring the difference in absorbance during the reaction, while the end point reaction method measures the total amount of substrate/products at the end of the reaction. Both methods are useful in enzyme analysis and are commonly used in clinical chemistry.