What is the Difference Between CHO-S and CHO-K1?
🆚 Go to Comparative Table 🆚CHO-S and CHO-K1 are both derived from the Chinese Hamster Ovary (CHO) original cell line, but they have distinct characteristics and applications. The main differences between CHO-S and CHO-K1 are:
- Cell Growth: CHO-S is a suspension cell line, while CHO-K1 can grow as either a suspension or adherent cell line.
- Glycine Biosynthesis: CHO-K1 is missing a chromosome that carries a gene necessary for glycine biosynthesis, so proline needs to be added to the media when culturing CHO-K1 cells.
- Application: CHO-S is ideal for large-scale bioreactors due to its ability to grow in suspension liquid culture. On the other hand, CHO-K1 is more suitable for gene expression studies.
- Bioprocessing: CHO-S has a preference for biomass formation, while CHO-K1 favors monoclonal antibody (mAb) production.
Despite these differences, both CHO-S and CHO-K1 cell lines are widely used in biological and medical research studies, as well as in commercially available therapeutic applications.
Comparative Table: CHO-S vs CHO-K1
CHO-S and CHO-K1 are both cell lines derived from Chinese Hamster Ovary (CHO) epithelial cells, but they have distinct characteristics and applications. Here is a table summarizing the differences between CHO-S and CHO-K1:
| Feature | CHO-S | CHO-K1 |
|---|---|---|
| Cell Type | Suspension cell line | Suspension or adherent cell line |
| Proline Synthesis | Proline is not added to the media when culturing | Proline is added to the media when culturing due to the lack of a proline-synthesizing gene |
| Applications | Widely used in industrial bioreactor systems | Applications focus more on gene expression studies |
| Specific Productivity | Prefers biomass production with lower protein expression | Favors cell-specific productivity |
CHO-S cells are suspension cell lines, adapted for growth in suspension liquid cultures. In contrast, CHO-K1 cells can be genetically manipulated to grow as suspension cells or adherent cells. CHO-K1 cells lack a proline-synthesizing gene, so proline is added to the media when culturing. CHO-S cells are widely used in industrial bioreactor systems, while CHO-K1 cells are more suitable for gene expression studies.
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