What is the Difference Between 1D and 2D Gel Electrophoresis?
🆚 Go to Comparative Table 🆚The main difference between 1D and 2D gel electrophoresis lies in the properties used for protein separation. Here are the key differences between the two techniques:
1D Gel Electrophoresis:
- Separates proteins based on their molecular weight.
- Primarily uses polyacrylamide gel electrophoresis.
- Has a lower resolution compared to 2D gel electrophoresis.
- Less costly than 2D gel electrophoresis.
2D Gel Electrophoresis:
- Separates proteins based on their isoelectric point and molecular weight.
- Has a higher resolution compared to 1D gel electrophoresis.
- More costly than 1D gel electrophoresis.
In summary, 1D gel electrophoresis separates proteins based on their molecular weight using polyacrylamide gels, while 2D gel electrophoresis separates proteins based on both their isoelectric point and molecular weight. Although 2D gel electrophoresis offers higher resolution, it is more expensive than 1D gel electrophoresis.
Comparative Table: 1D vs 2D Gel Electrophoresis
Here is a table comparing the differences between 1D and 2D gel electrophoresis:
| Feature | 1D Gel Electrophoresis | 2D Gel Electrophoresis |
|---|---|---|
| Separation | Based on molecular weight | Based on isoelectric point and molecular weight |
| Dimensions | One-dimensional | Two-dimensional |
| Technique | Polyacrylamide gel electrophoresis | Combination of isoelectric focusing (IEF) and SDS-PAGE |
| Resolution | Lower resolution | Higher resolution |
| Protein Sample | Separates proteins according to their size and charge | Separates proteins according to their isoelectric point and molecular weight |
| Application in Proteomics | Less commonly used | Primary technique in proteomics |
| Cost | Less expensive | More expensive |
1D gel electrophoresis separates proteins based on their molecular weight, primarily using polyacrylamide gel electrophoresis. In contrast, 2D gel electrophoresis separates proteins based on both their isoelectric point and molecular weight, using a combination of isoelectric focusing (IEF) and SDS-PAGE. 2D gel electrophoresis has a higher resolution and is more commonly used in proteomics research, but it is also more expensive than 1D gel electrophoresis.
- Gel vs Paper Electrophoresis
- Capillary Electrophoresis vs Gel Electrophoresis
- Horizontal vs Vertical Gel Electrophoresis
- Electrophoresis vs Dielectrophoresis
- Gel Electrophoresis vs SDS Page
- Native vs Denaturing Gel Electrophoresis
- Agarose vs Polyacrylamide Gel Electrophoresis
- Electrophoresis vs Chromatography
- Electrophoresis vs Electroosmosis
- Electrophoretic vs Asymmetric Effect
- Gel Filtration vs Gel Permeation Chromatography
- Electrophoretic Deposition vs Electrodeposition
- Stacking Gel vs Separating Gel
- Gel Filtration vs Affinity Chromatography
- Metaphase 1 vs 2
- 2D vs 3D Cell Culture
- Differential vs Density Gradient Centrifugation
- First vs Second Ionization Energy (I1E vs I2E)
- Agar vs Agarose