What is the Difference Between 16s rRNA and 16s rDNA?

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The main difference between 16S rRNA and 16S rDNA lies in their structure and function. Here are the key differences:

  1. Structure: 16S rRNA is a single-stranded ribosomal RNA that forms part of the 30S subunit in prokaryotic ribosomes. In contrast, 16S rDNA is the double-stranded chromosomal DNA that codes for the 16S rRNA.
  2. Function: 16S rRNA plays a crucial role in protein synthesis within the ribosomes of prokaryotes. On the other hand, 16S rDNA is the gene responsible for producing 16S rRNA through the process of transcription.
  3. Molecular Markers: 16S rDNA is considered a universal sequence in bacteria and is highly conserved across various species. As a result, it serves as a molecular marker in phylogenetic studies, helping to determine relationships between different bacterial species.

In summary, 16S rRNA is a single-stranded RNA involved in protein synthesis, while 16S rDNA is the double-stranded DNA gene that codes for the 16S rRNA. Both play essential roles in the functioning of prokaryotic cells and are widely used in microbiological studies for identification and classification purposes.

Comparative Table: 16s rRNA vs 16s rDNA

Here is a table summarizing the differences between 16s rRNA and 16s rDNA:

Feature 16s rRNA 16s rDNA
Composition Single-stranded RNA Double-stranded DNA
Function Forms a component of the 30s subunit in prokaryotic ribosomes Encodes the 16s rRNA sequence
Size Approximately 1550 bp Contains both non-coding and coding strands
Bases Contains nucleotide sequences Contains thymine bases in its nucleotide sequences
Synthesis Transcribed from 16s rDNA Found in the genome of prokaryotes

In summary, 16s rRNA is a single-stranded ribosomal RNA that forms part of the 30s subunit in prokaryotic ribosomes, while 16s rDNA is the double-stranded chromosomal DNA encoding the 16s rRNA sequence. The transcription of the 16s rDNA gene gives the 16s rRNA sequence. Both 16s rRNA and 16s rDNA are involved in protein synthesis in prokaryotes and are considered molecular markers for phylogenetic studies due to their highly conserved nature in various species.